Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human UGT1A1 293 Cell Lysate

Cat.No. : UGT1A1-513HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for UDP glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : UGT1A1 UDP glucuronosyltransferase 1 family, polypeptide A1 [ Homo sapiens ]
Official Symbol : UGT1A1
Synonyms : UGT1A1; UDP glucuronosyltransferase 1 family, polypeptide A1; GNT1, UDP glycosyltransferase 1 family, polypeptide A1 , UGT1; UDP-glucuronosyltransferase 1-1; UGT1A; UGT-1A; UGT1*1; UGT1.1; UGT1-01; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; bilirubin-specific UDPGT isozyme 1; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; UDP glycosyltransferase 1 family, polypeptide A1; GNT1; UGT1; UDPGT; HUG-BR1; BILIQTL1; UDPGT 1-1;
Gene ID : 54658
mRNA Refseq : NM_000463
Protein Refseq : NP_000454
MIM : 191740
UniProt ID : P22309
Chromosome Location : 2q37
Pathway : Ascorbate and aldarate metabolism, organism-specific biosystem; Ascorbate and aldarate metabolism, conserved biosystem; Ascorbate biosynthesis, animals, glucose-1P =>ascorbate, organism-specific biosystem; Ascorbate biosynthesis, animals, glucose-1P => ascorbate, conserved biosystem; Biological oxidations, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
What is the function of UGT1A1? 07/14/2022

UGT1A1 converts lipophilic small molecules, such as drugs, into water-soluble and excretable metabolites.

What is the relationship between bilirubin and UGT1A1? 01/24/2022

Bilirubin is a binding substrate for UGT1A1 and UGT1A1 is involved in bilirubin metabolism.

What diseases is UGT1A1 involved in? 11/23/2021

UGT1A1 is involved in lung cancer, colorectal cancer and hyperbilirubinemia, among other diseases.

Where is UGT1A1 mainly expressed? 03/11/2021

UGT1A1 is mainly expressed in the liver.

What are the effects of mutations in the UGT1A1 gene? 02/09/2021

Mutations in the UGT1A1 gene prevent the glucuronidation of free bilirubin and reduces bilirubin metabolism and increases serum free bilirubin levels.

Customer Reviews (2)

Write a review
Reviews
08/12/2022

    The product was kept in perfect condition and delivered on time so that I got the perfect product in the shortest time possible.

    05/14/2022

      Customer service contacted me in a timely manner and helped me solve a lot of problems that arose in my experiments.

      Ask a Question for All UGT1A1 Products

      Required fields are marked with *

      My Review for All UGT1A1 Products

      Required fields are marked with *

      0

      Inquiry Basket

      cartIcon
      logo

      FOLLOW US

      Terms and Conditions        Privacy Policy

      Copyright © 2024 Creative BioMart. All Rights Reserved.

      Contact Us

      • /

      Stay Updated on the Latest Bioscience Trends