Recombinant Human UGT1A4 Protein (29-534 aa), His-tagged UGT1A4-2109H

Recombinant Human UGT1A4 Protein (29-534 aa), His-tagged

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Recombinant Human UGT1A4 Protein (29-534 aa), His-tagged

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Cat.No. : UGT1A4-2109H
Product Overview : Recombinant Human UGT1A4 Protein (29-534 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length of Mature Protein.
Description : UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1 (By similarity).
Source : E. coli
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 61.0 kDa
Protein length : 29-534 aa
AA Sequence : GKVLVVPTDGSPWLS MREALRELHARGHQA VVLTPEVNMHIKEEK FFTLTAYAVPWTQKE FDRVTLGYTQGFFET EHLLKRYSRSMAIMN NVSLALHRCCVELLH NEALIRHLNATSFDV VLTDPVNLCGAVLAK YLSIPAVFFWRYIPC DLDFKGTQCPNPSSY IPKLLTTNSDHMTFL QRVKNMLYPLALSYI CHTFSAPYASLASEL FQREVSVVDLVSYAS VWLFRGDFVMDYPRP IMPNMVFIGGINCAN GKPLSQEFEAYINAS GEHGIVVFSLGSMVS EIPEKKAMAIADALG KIPQTVLWRYTGTRP SNLANNTILVKWLPQ NDLLGHPMTRAFITH AGSHGVYESICNGVP MVMMPLFGDQMDNAK RMETKGAGVTLNVLE MTSEDLENALKAVIN DKSYKENIMRLSSLH KDRPVEPLDLAVFWV EFVMRHKGAPHLRPA AHDLTWYQYHSLDVI GFLLAVVLTVAFITF KCCAYGYRKCLGKKG RVKKAHKSKTH
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : UGT1A4 UDP glucuronosyltransferase 1 family, polypeptide A4 [ Homo sapiens ]
Official Symbol : UGT1A4
Synonyms : UGT1A4; HUG BR2; UGT1D; UDPGT; UGT-1D; UGT1.4; HUG-BR2; UGT1-04; UDPGT 1-4;
Gene ID : 54657
mRNA Refseq : NM_007120
Protein Refseq : NP_009051
MIM : 606429
UniProt ID : P22310

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