Recombinant Human UMPS Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UMPS-3314H
• Product Overview: UMPS MS Standard C13 and N15-labeled recombinant protein (NP_000364) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 52.2 kDa
• AA Sequence: MAVARAALGPLVTGLYDVQAFKFGD FVLKSGLSSPIYIDLRGIVSRPRLL SQVADILFQTAQNAGISFDTVCGVP YTALPLATVICSTNQIPMLIRRKET KDYGTKRLVEGTINPGETCLIIEDV VTSGSSVLETVEVLQKEGLKVTDAI VLLDREQGGKDKLQAHGIRLHSVCT LSKMLEILEQQKKVDAETVGRVKRF IQENVFVAANHNGSPLSIKEAPKEL SFGARAELPRIHPVASKLLRLMQKK ETNLCLSADVSLARELLQLADALGP SICMLKTHVDILNDFTLDVMKELIT LAKCHEFLIFEDRKFADIGNTVKKQ YEGGIFKIASWADLVNAHVVPGSGV VKGLQEVGLPLHRGCLLIAEMSSTG SLATGDYTRAAVRMAEEHSEFVVGF ISGSRVSMKPEFLHLTPGVQLEAGG DNLGQQYNSPQEVIGKRGSDIIIVG RGIISAADRLEAAEMYRKAAWEAYL SRLGVTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UMPS uridine monophosphate synthetase [ Homo sapiens (human) ]
• Official Symbol: UMPS
• Synonyms: UMPS; uridine monophosphate synthetase; uridine 5-monophosphate synthase; orotate phosphoribosyl transferase and orotidine 5 decarboxylase; OPRTase; OMPdecase; UMP synthase; orotate phosphoribosyltransferase; orotidine 5-phosphate decarboxylase; OPRT
• Gene ID: 7372
• mRNA Refseq: NM_000373
• Protein Refseq: NP_000364
• MIM: 613891
• UniProt ID: P11172