"VCAM1" Related Products

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Recombinant Human VCAM1 protein, His-tagged

Cat.No.: VCAM1-170H
Product Overview: Recombinant Human VCAM1(Phe25-Glu698) fused with His tag at the C-terminus was expressed in NS0.
Source: NS0
Species: Human
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Predicted Molecular Mass: 101.6 (monomer) kDa;SDS-PAGE: 130-140 kDa, reducing conditions
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
Gene Name: VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens ]
Official Symbol: VCAM1
Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; CD106; CD106 antigen; INCAM-100; MGC99561; DKFZp779G2333;
Gene ID: 7412
mRNA Refseq: NM_001078
Protein Refseq: NP_001069
MIM: 192225
UniProt ID: P19320
Chromosome Location: 1p32-p31
Pathway: Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function: cell adhesion molecule binding; cell adhesion molecule binding; integrin binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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