Active Recombinant Human VCAM1 protein, His-tagged

• Cat.No.: VCAM1-3371H
• Product Overview: Active Recombinant Human VCAM1 protein(P19320)(109~318 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 109-318 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Vascular cell adhesion protein 1 (VCAM-1) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. Besides, Integrin Alpha 4 (ITGa4) has been identified as an interactor of VCAM-1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VCAM-1 and recombinant human ITGa4. Briefly, biotin-linked VCAM-1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGa4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human VCAM-1 and recombinant human ITGa4 was shown, the EC50 for this effect is 0.261 ug/mL.
• Molecular Mass: 25.0kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: VCAM1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; CD106; CD106 antigen; INCAM-100; MGC99561; DKFZp779G2333
• Gene ID: 7412
• mRNA Refseq: NM_001078
• Protein Refseq: NP_001069
• MIM: 192225
• UniProt ID: P19320