|Product Overview:||Recombinant human VEGFA (Ala27-Arg171) protein without tag was expressed in E. coli.|
|Bio-activity:||Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 12-60 ng/mL.|
|Molecular Mass:||Predicted Molecular Mass: 17 kDa (monomer)
SDS-PAGE: 20 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-Ala27 & Pro28|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer:||Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.|
|Reconstitution:||Reconstitute at 100 μg/mL 4 mM HCl containing at least 0.1% human or bovine serum albumin.|
|Gene Name:||VEGFA vascular endothelial growth factor A [ Homo sapiens (human) ]|
|Synonyms:||MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor|