Recombinant Human VEGFA Protein, His-Avi-tagged, Biotinylated

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Recombinant Human VEGFA Protein, His-Avi-tagged, Biotinylated

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Cat.No. : VEGFA-274H
Product Overview : Biotinylated Human VEGF121, His Tag is expressed from HEK293 cells. It contains AA Ala 27 - Arg 147 (Accession # P15692-9). This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.
Source : HEK293
Species : Human
Tag : His-Avi
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized VEGFR1/R2-Fc at 1ug/mL (100 uL/well) can bind Biotinylated Human VEGF121, His Tag with a linear range of 0.1-2 ug/mL.
Molecular Mass : The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa on a SDS-PAGE gel under reducing condition and 35-40 kDa under non-reducing condition.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE..
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : VEGFA vascular endothelial growth factor A [ Homo sapiens ]
Official Symbol : VEGFA
Synonyms : VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609;
Gene ID : 7422
mRNA Refseq : NM_001025366
Protein Refseq : NP_001020537
UniProt ID : P15692

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