Active Recombinant Human VSIG2 Protein, His-tagged

• Cat.No.: VSIG2-3911H
• Product Overview: Active Recombinant Human VSIG2 Protein(Q96IQ7-1)(Met 1-Ala 243) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-243 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to human VSIG2 coated plates (30 μg/ml, 100 μl/well), approximately 25 %-50 % will adhere after one hour at 37 °C.
• Molecular Mass: The secreted recombinant human VSIG2 consists of 231 amino acids and has a calculated molecular mass of 24.6 kDa. The apparent molecular mass of rhVSIG2 is approximately 30-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VSIG2 V-set and immunoglobulin domain containing 2 [ Homo sapiens ]
• Official Symbol: VSIG2
• Synonyms: VSIG2; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; CTH; CTXL; CT-like protein; cortical thymocyte-like protein; cortical thymocyte receptor (X. laevis CTX) like; 2210413P10Rik
• Gene ID: 23584
• mRNA Refseq: NM_014312
• Protein Refseq: NP_055127
• MIM: 606011
• UniProt ID: Q96IQ7