Recombinant Human VTCN1 Protein, His-tagged, Biotinylated

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Recombinant Human VTCN1 Protein, His-tagged, Biotinylated

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Cat.No. : VTCN1-429H
Product Overview : Biotinylated Human B7-H4 is expressed from HEK293 cells. It contains AA Phe 29 - Ala 258 (Accession # NP_078902). This protein carries a polyhistidine tag at the C-terminus.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Phe 29
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized anti-B7-H4 mAb (Human IgG1) at 2 ug/mL can bind Biotinylated Human B7-H4 with a linear range of 4-62 ug/mL.
Molecular Mass : The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 0.7-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : VTCN1 V-set domain containing T cell activation inhibitor 1 [ Homo sapiens ]
Official Symbol : VTCN1
Synonyms : VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4;
Gene ID : 79679
mRNA Refseq : NM_001253849
Protein Refseq : NP_001240778
MIM : 608162
UniProt ID : Q7Z7D3
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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