Recombinant Human VWF, His tagged

• Cat.No.: VWF-894H
• Product Overview: Recombinant Human VWF pro form (NP_000543.2) (Met 1-Lys 2813), fused with a C-terminal polyhistidine tag, was produced in CHO Cells.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Met 1-Lys 2813
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom (2018)

Gene Information

• Gene Name: VWF von Willebrand factor [ Homo sapiens ]
• Official Symbol: VWF
• Synonyms: VWF; von Willebrand factor; F8VWF; coagulation factor VIII VWF; VWD
• Gene ID: 7450
• mRNA Refseq: NM_000552
• Protein Refseq: NP_000543
• MIM: 613160
• UniProt ID: P04275

Citation

Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra ( Naja kaouthia ) Venom

Journal: Toxins    PubMed ID: 30513883    Data: 2018/12/3

Authors: Jirawat Khanongnoi, Siratcha Phanthong, Nitat Sookrung

Article Snippet:Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.. The presence of kaouthiagin in individual fractions were determined by probing the SDS-PAGE-separated fractions with 6× His-tagged-recombinant human vWF (r-hvWF) (VWF-894H, Creative BioMart, Ramsey road, Shirley, NY, USA), followed by mouse anti-6× His antibody, goat-anti-mouse IgG-alkaline phosphatase (AP) conjugate (Southern Biotech, Birmingham, AL, USA) and AP substrate (BCIP/NBT; KPL, Seracare Life Sciences, Milford, MA, USA), respectively, with washing and incubation between the steps.. The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.