Recombinant Human Full Length VWF protein, His tagged

• Cat.No.: VWF-894H
• Product Overview: Recombinant Human Full Length VWF protein(NP_000543.2) (Met 1-Lys 2813) was expressed in CHO with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 1-2813 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the form with the peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000543.2
• Unit ID: P04275
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P04275
• Publications:
  • Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom (2018)
  • Concomitant Acquired Hemophilia A and Acquired Von Willebrand Syndrome from Distinctive Autoantibodies: Case Report (2025)

Gene Information

• Gene Name: VWF von Willebrand factor [ Homo sapiens ]
• Official Symbol: VWF
• Synonyms: VWF; von Willebrand factor; F8VWF; coagulation factor VIII VWF; VWD
• Gene ID: 7450
• mRNA Refseq: NM_000552
• protein Refseq: NP_000543
• MIM: 613160

Citation

Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra ( Naja kaouthia ) Venom

Journal: Toxins    PubMed ID: 30513883    Data: 2018/12/3

Authors: Jirawat Khanongnoi, Siratcha Phanthong, Nitat Sookrung

Article Snippet:Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.. The presence of kaouthiagin in individual fractions were determined by probing the SDS-PAGE-separated fractions with 6× His-tagged-recombinant human vWF (r-hvWF) (VWF-894H, Creative BioMart, Ramsey road, Shirley, NY, USA), followed by mouse anti-6× His antibody, goat-anti-mouse IgG-alkaline phosphatase (AP) conjugate (Southern Biotech, Birmingham, AL, USA) and AP substrate (BCIP/NBT; KPL, Seracare Life Sciences, Milford, MA, USA), respectively, with washing and incubation between the steps.. The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.