Active Recombinant Human Full Length VWF protein, His tagged
Cat.No. : | VWF-894H |
Product Overview : | Active Recombinant Human Full Length VWF protein(AAB59458.1) (Met1-Lys2813) was expressed in CHO with a C-terminal polyhistidine tag. |
Availability | July 30, 2025 |
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Species : | Human |
Source : | CHO |
Tag : | His |
Protein Length : | 1-2813 aa |
Form : | Lyophilized from sterile PBS, pH 7.4. |
Bio-activity : | Immobilized BDD-FVIII at 2 μg/mL (100 μL/well) can bind Recombinant Human vWF Protein (His Tag), with a linear range of 0.16-4.0 μg/mL. |
Molecular Mass : | The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | ≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Publications : |
Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom (2018)
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Gene Name | VWF von Willebrand factor [ Homo sapiens ] |
Official Symbol | VWF |
Synonyms | VWF; von Willebrand factor; F8VWF; coagulation factor VIII VWF; VWD; |
Gene ID | 7450 |
mRNA Refseq | NM_000552 |
Protein Refseq | NP_000543 |
MIM | 613160 |
UniProt ID | P04275 |
◆ Recombinant Proteins | ||
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VWF-5257B | Recombinant Bovine VWF protein | +Inquiry |
VWF-2643M | Recombinant Mouse VWF Protein (1498-1665 aa), His-Myc-tagged | +Inquiry |
VWF-5258B | Recombinant Bovine VWF protein | +Inquiry |
◆ Native Proteins | ||
VWF-369H | Native Human Von Willebrand Factor | +Inquiry |
VWF-17H | Native Human von Willebrand Factor, Factor VIII Free | +Inquiry |
◆ Cell & Tissue Lysates | ||
VWF-001HCL | Recombinant Human VWF cell lysate | +Inquiry |
Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra ( Naja kaouthia ) Venom
Journal: Toxins PubMed ID: 30513883 Data: 2018/12/3
Authors: Jirawat Khanongnoi, Siratcha Phanthong, Nitat Sookrung
Article Snippet:Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.Protein concentrations of all eluted fractions were measured and analyzed by SDS-PAGE and Coomassie Brilliant Blue G-250 (CBB) staining.. The presence of kaouthiagin in individual fractions were determined by probing the SDS-PAGE-separated fractions with 6× His-tagged-recombinant human vWF (r-hvWF) (VWF-894H, Creative BioMart, Ramsey road, Shirley, NY, USA), followed by mouse anti-6× His antibody, goat-anti-mouse IgG-alkaline phosphatase (AP) conjugate (Southern Biotech, Birmingham, AL, USA) and AP substrate (BCIP/NBT; KPL, Seracare Life Sciences, Milford, MA, USA), respectively, with washing and incubation between the steps.. The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.The r-hvWF-bound fractions were collected, dialyzed against distilled water, concentrated and subjected to LC-MS/MS for kaouthiagin verification.
Not For Human Consumption!
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