Recombinant Human WDR5 293 Cell Lysate

Cat.No. : WDR5-343HCL
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Description : Antigen standard for WD repeat domain 5 (WDR5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : WDR5 WD repeat domain 5 [ Homo sapiens ]
Official Symbol : WDR5
Synonyms : WDR5; WD repeat domain 5; WD repeat-containing protein 5; SWD3; Set1c WD40 repeat protein; homolog (S. cerevisiae); WD-repeat protein 5; BMP2-induced 3-kb gene protein; SWD3, Set1c WD40 repeat protein, homolog; BIG-3;
Gene ID : 11091
mRNA Refseq : NM_052821
Protein Refseq : ?NP_438172
MIM : 609012
UniProt ID : P61964
Chromosome Location : 9q34
Pathway : Circadian rhythm pathway, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
11/25/2020

    Their ability to accommodate the requirements of large-scale experiments and guarantee a reliable supply streamlines my research operations, eliminating any concerns related to potential shortages.

    10/10/2020

      I am confident that the protein will reliably perform in my assays, providing accurate and reproducible results.

      09/25/2017

        the manufacturer's supply management capabilities assure a seamless and continuous provision of the WDR5 protein.

        Q&As (5)

        Ask a question
        Can WDR5 play a role in non-cancerous diseases? 03/25/2023

        Yes, WDR5 has been associated with neurodevelopmental disorders, suggesting a role in non-cancerous diseases as well.

        How does WDR5 affect neurodevelopment? 11/09/2021

        WDR5 regulates the expression of genes critical for neural development, and dysregulation can lead to developmental disorders.

        How can WDR5 be used as a diagnostic marker for cancer? 03/02/2019

        Elevated levels of WDR5 in certain cancers can serve as a diagnostic marker when detected in patient samples.

        Are there any clinical trials involving WDR5-targeted therapies? 02/25/2018

        Yes, there are ongoing clinical trials to test the safety and efficacy of WDR5-targeted therapies in cancer patients.

        Is there a connection between WDR5 and autoimmune diseases? 03/29/2017

        Some studies suggest a potential link between WDR5 and autoimmune diseases, but further research is needed to confirm this association.

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