Recombinant Human XRCC4 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: XRCC4-078H
• Product Overview: XRCC4 MS Standard C13 and N15-labeled recombinant protein (NP_072044) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 37.9 kDa
• AA Sequence: MERKISRIHLVSEPSITHFLQVSWE KTLESGFVITLTDGHSAWTGTVSES EISQEADDMAMEKGKYVGELRKALL SGAGPADVYTFNFSKESCYFFFEKN LKDVSFRLGSFNLEKVENPAEVIRE LICYCLDTIAENQAKNEHLQKENER LLRDWNDVQGRFEKCVSAKEALETD LYKRFILVLNEKKTKIRSLHNKLLN AAQEREKDIKQEGETAICSEMTADR DPVYDESTDEESENQTDLSGLASAA VSKDDSIISSLDVTDIAPSRKRRQR MQRNLGTEPKMAPQENQLQEKENSR PDSSLPETSKKEHISAENMSLETLR NSSPEDLFDEITRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: XRCC4 X-ray repair cross complementing 4 [ Homo sapiens (human) ]
• Official Symbol: XRCC4
• Synonyms: XRCC4; X-ray repair cross complementing 4; SSMED; DNA repair protein XRCC4; X-ray repair complementing defective repair in Chinese hamster cells 4
• Gene ID: 7518
• mRNA Refseq: NM_022550
• Protein Refseq: NP_072044
• MIM: 194363
• UniProt ID: Q13426