Recombinant Mouse Abat protein, His & T7-tagged

• Cat.No.: Abat-1035M
• Product Overview: Recombinant Mouse Abat aa. (Ile236~Lys500 (Accession # P61922) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The encoded gene product is responsible for catabolism of gamma-aminobutyric acid (GABA), a mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. Deficiency of this encoded protein includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.9kDa.
• Protein length: Ile236~Lys500 (Accession # P61922)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Abat 4-aminobutyrate aminotransferase [ Mus musculus (house mouse) ]
• Official Symbol: Abat
• Synonyms: Abat; 4-aminobutyrate aminotransferase; I54; Gabat; Gabaat; Gm9851; Laibat; X61497; AI255750; 9630038C02Rik; 4-aminobutyrate aminotransferase, mitochondrial; (S)-3-amino-2-methylpropionate transaminase; 4-aminobutyrate transaminase; GABA aminotransferase; GABA transaminase; GABA transferase; beta-alanine oxoglutarate aminotransferase; gamma-amino-N-butyrate transaminase
• Gene ID: 268860
• mRNA Refseq: NM_001170978.1
• Protein Refseq: NP_001164449.1
• UniProt ID: P61922