Recombinant Mouse Abcd4 protein, His & T7-tagged
|Product Overview :||Recombinant Mouse Abcd4 aa. (Arg356~Glu606 (Accession # O89016)) fused with N-terminal His & T7 tag was produced in E. coli cells.|
- Gene Information
- Related Products
|Source :||E. coli|
|Tag :||His & T7|
|Form :||Freeze-dried powder|
|Molecular Mass :||Predicted Molecular Mass: 31.6kDa.|
|Protein length :||Arg356~Glu606 (Accession # O89016)|
|Endotoxin :||<1.0EU per 1ug (determined by the LAL method)|
|Characteristic :||The isoelectric point is 5.|
|Applications :||SDS-PAGE; WB; ELISA; IP.|
|Stability :||The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.|
|Storage :||Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.|
|Storage Buffer :||Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.|
|Reconstitution :||Reconstitute in sterile ddH2O.|
|Gene Name :||Abcd4 ATP-binding cassette, sub-family D (ALD), member 4 [ Mus musculus (house mouse) ]|
|Official Symbol :||Abcd4|
|Synonyms :||Abcd4; ATP-binding cassette, sub-family D (ALD), member 4; P69r; P70R; Pxmp1l; ATP-binding cassette sub-family D member 4; PMP69; PMP70-related protein; PXMP1-L; peroxisomal membrane protein 69; peroxisomal membrane protein, 70 kDa-related|
|Gene ID :||19300|
|mRNA Refseq :||NM_008992.2|
|Protein Refseq :||NP_033018.2|
|UniProt ID :||O89016|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (21)Ask a question
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Mutations in the ABCD4 gene can lead to decreased levels of vitamin B12, as its transport into the peroxisomes is impaired.
ABCD4 is involved in the transport of vitamin B12 from the cytoplasm into the peroxisomes, where it is metabolized.
Mutations in the ABCD4 gene are associated with a disorder called ABCD4-related disease, which affects the metabolism of vitamin B12.
ABCD4 is synthesized in the endoplasmic reticulum (ER) and subsequently targeted to the peroxisomal membrane through specific trafficking pathways.
ABCD4 plays a significant role in specific metabolic pathways and cellular processes related to cobalamin (vitamin B12) metabolism, including cobalamin transport, methionine and S-adenosylmethionine synthesis, Methylmalonyl-CoA mutase pathway and development of nervous system.
Yes, ABCD4 mutations can be inherited in an autosomal recessive manner, meaning both parents must carry a mutated copy of the gene for the disorder to manifest in their offspring.
ABCD4 is a transmembrane protein that consists of multiple domains, including transmembrane domains and ATP-binding domains.
Yes, alterations in ABCD4 expression levels can impact cellular metabolism, particularly fatty acid metabolism and the processing of vitamin B12.
In some cases, ABCD4 dysfunction can result in developmental abnormalities, particularly affecting the nervous system due to impaired vitamin B12 metabolism.
ABCD4 protein primarily locate in endoplasmic reticulum membrane and lysosome membrane.
ABCD4 protein is popular present in human, mouse, zebrafish, ecoli, bovine and rice.
The ABCD4 gene contains various regulatory elements and sequences that control its expression, such as promoter regions, enhancers, and transcription factor binding sites.
According to the record, ABCD4 is a kind of lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner
Mutations in the ABCD4 gene may lead to a range of congenital vitamin B12 deficiency diseases, which may present with hypotonia, myelosuppression, macrocytic anemia, and heart disease
ABCD4 expression is found in various tissues, but its levels can vary, with higher expression observed in tissues involved in fatty acid metabolism and vitamin B12 transport.
ABCD4 is known to interact with and transport cobalam, playing a critical role in the intracellular transport of cobalamin across cellular membranes, particularly in lysosomes and peroxisomes.
ATP-binding domains (NBDs) and Transmembrane domains (TMDs) are two key domains found in ABCD4.
Yes, animal models such as mice have been developed to study ABCD4-related disease and gain insights into its pathophysiology.
Yes, the manifestation of ABCD4-related disease can vary among individuals, leading to a spectrum of symptoms and severity.
ABCD4 Interacts with LMBRD1 and this interaction induces the translocation of ABCD4 from the ER to the lysosome membrane
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It made it convenient to prepare my experimental solutions. -
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It had exceptional purity. -
The functional study involving the protein product demonstrated the expected effects -
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