Recombinant Mouse Actb Protein, MYC/DDK-tagged

• Cat.No.: Actb-812M
• Product Overview: Purified recombinant protein of full-length mouse actin, beta (Actb), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the actin family of proteins. Actins are highly conserved proteins that are among the most abundant proteins in eukaryotic cells and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the transcribed mRNA are regulated through the binding of multiple factors to its 3' UTR sequence. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous pseudogenes of this gene have been identified in the mouse genome.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 41.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Actb actin, beta [ Mus musculus (house mouse) ]
• Official Symbol: Actb
• Synonyms: Actb; actin, beta; Actx; beta-actin; E430023M04Rik; actin, cytoplasmic 1; A-X actin-like protein; actin, beta, cytoplasmic; melanoma X-actin
• Gene ID: 11461
• mRNA Refseq: NM_007393
• Protein Refseq: NP_031419
• UniProt ID: P60710

Reviews

08/19/2022

I could trust the product's precision, ensuring reliable data generation

08/15/2021

The protein product had an impressive shelf life, maintaining its quality and functionality over an extended period

03/04/2021

The protein product seamlessly integrated with various experimental techniques, enhancing its versatility

02/23/2020

The protein product underwent rigorous safety testing, ensuring its reliability and suitability for experimentation

06/19/2018

The protein product line offered a wide variety of options for different research need

Q&As

Which biological process GO terms were identified through the PPI analysis?

 The PPI analysis identified several biological process GO terms such as "Actin cytoskeleton organization," "Box C/D snoRNP assembly," "Actin filament depolymerization," "Cell junction assembly," and "DNA repair."

What was the focus of the current study mentioned?

The current study aimed to analyze the diagnostic value of ACTB and predict its independent prognostic factor in distinct cancer subtypes using detailed in silico analysis.

How could ACTB potentially serve as a biomarker in LIHC, HNSC, and LUAD?

The findings suggest that due to its elevated expression, association with poorer survival and metastasis, and correlation with important parameters such as immune infiltration and tumor purity, ACTB has the potential to be a candidate biomarker for LIHC, HNSC, and LUAD.

How is ACTB related to the metastasis of different cancers?

The dysregulation and polymerization of ACTB have been revealed to be associated with the metastasis of different cancer types.

Which drugs were found to reduce the expression level of ACTB?

Warfarin and aflatoxin B1 were found to potentially reduce the expression level of ACTB.

How many highly significant transcription factors (TFs) were identified as potential regulators of ACTB expression?

Ten highly significant TFs were identified as potential regulators of ACTB expression. They are AATF, WWTR1, GFI1, NR3C1, MYC, GFI1, STAT3, ING1, MAX, and POU4F1.

What do the results suggest about the regulation of ACTB expression?

The results indicate that a variety of different regulators, including miRNAs and TFs, are involved in the regulation of ACTB expression.

What analytical method was used to identify potential regulators of ACTB expression?

Enrichr analysis was used to identify potential regulators of ACTB expression.

What molecular functions were associated with the ACTB-associated genes?

The molecular functions associated with the ACTB-associated genes included "Actin monomer binding," "ATPase activity," "Leucine zipper domain binding," and "DNA helicase activity."

Which drugs were found to elevate the expression level of ACTB?

Valporic acid and metribolone were found to potentially elevate the expression level of ACTB.

What is the significance of ACTB (eta-actin) as a housekeeping gene?

ACTB serves as a housekeeping gene commonly used as a control in measuring gene expression in various diseases.

How many miRNAs were found to have the potential to change ACTB expression?

Ten miRNAs were identified as potential regulators of ACTB expression. They are hsa-miR-1908, hsa-miR-663, hsa-miR-744, hsa-miR-4745-3p, hsa-miR-1538, hsa-miR-3960, hsa-miR-4749-5p, hsa-miR-4706, hsa-miR-4743, and hsa-miR-3180-3p.

In which specific cancers was elevated ACTB expression associated with poorer survival and metastasis?

Elevated ACTB expression was associated with poorer survival and metastasis specifically in liver hepatocellular carcinoma (LIHC), head and neck squamous cancer (HNSC), and lung adenocarcinoma (LUAD).

What did the protein-protein interaction (PPI) analysis reveal about ACTB-associated genes?

The PPI analysis revealed a set of ten ACTB-associated genes that are involved in various biological processes, including "Actin cytoskeleton organization," "Box C/D snoRNP assembly," "Actin filament depolymerization," "Cell junction assembly," and "DNA repair."

What analytical approach was used in the study?

The study utilized in silico analysis to assess the diagnostic value and independent prognostic factor of ACTB in different cancer subtypes.

How was ACTB expression influenced through CTD analysis?

Through CTD analysis, it was observed that ACTB expression can potentially be influenced by a number of drugs.

Which cancers have been found to have a close association with ACTB expression?

 ACTB expression has been found to be closely related to various cancers, including liver, pancreatic, renal, colorectal, melanoma, prostate, esophageal, lung, gastric, breast, and ovarian cancers.

How was ACTB-associated expression regulated in the study?

The study explored ACTB-associated clinically important expression regulators, including TFs (transcription factors), miRNAs, and different chemotherapeutic drugs.

What was observed regarding ACTB expression in major human cancer tissues compared to normal samples?

ACTB expression was found to be remarkably higher in 24 major human cancer tissues compared to normal samples.

What other parameters did ACTB up-regulation show interesting correlations with?

ACTB up-regulation showed interesting correlations with immune infiltration of CD4+ T and CD8+ T cells, tumor purity, mutant genes, and other important parameters.