Recombinant Mouse Arfgap1 Protein, Myc/DDK-tagged

• Cat.No.: Arfgap1-1674M
• Product Overview: Purified recombinant protein of mouse full-length ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 45.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Arfgap1 ADP-ribosylation factor GTPase activating protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Arfgap1
• Synonyms: ARFGAP1; ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase-activating protein 1; ARF1 GAP; ARF GAP 1; ARF1-directed GTPase-activating protein; ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor 1 GTPase-activating protein; Arf1gap; AI115377
• Gene ID: 228998
• mRNA Refseq: NM_145760
• Protein Refseq: NP_665703
• UniProt ID: Q9EPJ9