| Description : |
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons. |
| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
C-Flag |
| Form : |
25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol. |
| Molecular Mass : |
111.9 kDa |
| AA Sequence : |
MSRIESLTRARIDRSKEQATKTREK EKMKEAKDARYTNGHLFTTISVSGM TMCYACNKSITAKEALICPT CNVTIHNRCKDTLANCTKVKQKQQK AALLRNNTALQSVSLRSKTTTRERP TSAIYPSDSFRQSLLGSRRG LSSLSLAKSVSTTNIAGHFNDESPL GLRQILSQSTDSLNMRNRTLSVESL IDEGVEVFYNELMSDFEMDE KDFEADSWSLAVDSSFLQQHKKEVM KKQDVIYELIQTELHHVRTLKIMTR LFRTGMLEELQMEPEVVQGL FPCVDELSDIHTRFLNQLLERRRQA LCPGSTRNFVIHRLGDLLISQFSGS NAEQMRKTYSEFCSRHTKAL KLYKELYARDKRFQQFIRKMTRSAV LKRHGVQECILLVTQRITKYPVLIN RILQNSHGVEEEYQDLASAL GLVKELLSNVDQDVHELEKEARLQE IYNRMDPRAQTPVPGKGPFGRDELL RRKLIHEGCLLWKTATGRFK DVLLLLMTDVLVFLQEKDQKYIFTS LDKPSVVSLQNLIVRDIANQAKGMF LISSGPPEMYEVHAASRDDR TTWIRVIQQSVRLCPSREDFPLIET EDKAYLRRIKTKLQQKNQALVELLQ KNVELFAEMVHFQALKAGFV GMPPPALPRGLFRLESFESLRGERL LKDALREVEGLKDLLLGPCVDLPTT SREPALPLDSDSGSCPGVTA NGEARTFNGSIELCRADSDSSQKDR NGNQLRSPQEEVLQPLINLYGLLHG LQAVVVQQERLMEALFPEGP ERWEKLSRANSRDGEAGRAAVASVT PEKQATELALLQRQHTLLQEELRRC QRLGEERATEAGSLEARLRE SEQARALLEREAEEIRRQLAALGQN EPLPAEAPWARRPLDPRRRSLPAGD ALYLSFNPPQPSRGHDRLDL PVTVRSLHRPFDDREAQELGSPEDR LQDSSDPDTGSEEEVSSRLSPPHSP RDFTRMQDIPEETESRDGEP
TASES TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Notes : |
For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage : |
Store at -80 centigrade after receiving vials. |
| Concentration : |
>0.05 μg/μL as determined by microplate BCA method. |
| Use/Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
