Recombinant Mouse Axl Protein, Myc/DDK-tagged

• Cat.No.: Axl-1792M
• Product Overview: Purified recombinant protein of mouse full-length AXL receptor tyrosine kinase (Axl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 98.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Axl AXL receptor tyrosine kinase [ Mus musculus (house mouse) ]
• Official Symbol: Axl
• Synonyms: AXL; AXL receptor tyrosine kinase; tyrosine-protein kinase receptor UFO; ufo oncogene homolog; adhesion-related kinase; Ark; Ufo; Tyro7; AI323647
• Gene ID: 26362
• mRNA Refseq: NM_009465
• Protein Refseq: NP_033491
• UniProt ID: Q00993