Recombinant Mouse B3galt5 Protein, Myc/DDK-tagged

• Cat.No.: B3galt5-1800M
• Product Overview: Purified recombinant protein of mouse full-length UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3galt5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Also catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing the glycolipid GB5, also known as the stage-specific embryonic antigen-3 (SSEA-3).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 36 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: B3galt5 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 [ Mus musculus (house mouse) ]
• Official Symbol: B3galt5
• Synonyms: AU045265; b3Galt-V; 1190002B21Rik
• Gene ID: 93961
• mRNA Refseq: NM_033149
• Protein Refseq: NP_149161
• UniProt ID: Q9JI67