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Recombinant Mouse Bcdin3d Protein, Myc/DDK-tagged

Cat.No. : Bcdin3d-1846M
Product Overview : Purified recombinant protein of mouse full-length BCDIN3 domain containing (Bcdin3d), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
  • Specification
  • Gene Information
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Description : O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 32 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Bcdin3d BCDIN3 domain containing [ Mus musculus (house mouse) ]
Official Symbol : Bcdin3d
Synonyms : Bcdin3d; BCDIN3 domain containing; AV138748; 4930556P03Rik; RNA 5'-monophosphate methyltransferase; BCDIN3 domain-containing protein; pre-miRNA 5'-monophosphate methyltransferase; probable methyltransferase BCDIN3D; EC 2.1.1.-
Gene ID : 75284
mRNA Refseq : NM_029236
Protein Refseq : NP_083512
UniProt ID : Q91YP1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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