Recombinant Mouse BNIP3 Protein (1-156 aa), His-tagged

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Recombinant Mouse BNIP3 Protein (1-156 aa), His-tagged

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Cat.No. : BNIP3-2403M
Product Overview : Recombinant Mouse BNIP3 Protein (1-156 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.
Description : Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Source : E. coli
Species : Mouse
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 21.6 kDa
Protein length : 1-156 aa
AA Sequence : MSQSGEENLQ GSWVELHFSN GNGSSVPASV SIYNGDMEKI LLDAQHESGR SSSKSSHCDS PPRSQTPQDT NRAEIDSHSF GEKNSTLSEE DYIERRREVE SILKKNSDWI WDWSSRPENI PPKEFLFKHP KRTATLSMRN TSVMKKGGIF SADFLK
Purity : > 85% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : Bnip3 BCL2/adenovirus E1B interacting protein 3 [ Mus musculus ]
Official Symbol : BNIP3
Synonyms : BNIP3; BCL2/adenovirus E1B interacting protein 3; Nip3;
Gene ID : 12176
mRNA Refseq : NM_009760
Protein Refseq : NP_033890
UniProt ID : O55003

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