Recombinant Mouse Car10 Protein, His-tagged


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Recombinant Mouse Car10 Protein, His-tagged

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Cat.No. : Car10-910M
Product Overview : Recombinant mouse Car10 (P61215) (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Gln 23
Form : Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.
Molecular Mass : The secreted recombinant mouse Car10 comprises 289 amino acids and has a calculated molecular mass of 33.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >97 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Car10 carbonic anhydrase 10 [ Mus musculus ]
Official Symbol : Car10
Synonyms : Ca10; BB085816; 2700029L05Rik; CA-RP X; CARP X; carbonic anhydrase-related protein X
Gene ID : 72605
mRNA Refseq : NM_028296
Protein Refseq : NP_082572
UniProt ID : P61215
Chromosome Location : 11; 11 C
Function : molecular_function

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