Recombinant Mouse Car10 Protein, His-tagged Car10-910M

Recombinant Mouse Car10 Protein, His-tagged

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Cat.No. :	Car10-910M
Product Overview :	Recombinant mouse Car10 (P61215) (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
Source :	HEK293
Species :	Mouse
Tag :	His
Predicted N Terminal :	Gln 23
Form :	Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity :	Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.
Molecular Mass :	The secreted recombinant mouse Car10 comprises 289 amino acids and has a calculated molecular mass of 33.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method.
Purity :	>97 % as determined by SDS-PAGE.
Stability :	Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage :	Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Gene Name :	Car10 carbonic anhydrase 10 [ Mus musculus ]
Official Symbol :	Car10
Synonyms :	Ca10; BB085816; 2700029L05Rik; CA-RP X; CARP X; carbonic anhydrase-related protein X
Gene ID :	72605
mRNA Refseq :	NM_028296
Protein Refseq :	NP_082572
MIM :
UniProt ID :	P61215
Chromosome Location :	11; 11 C
Function :	molecular_function