Recombinant Mouse Car12 Protein, Myc/DDK-tagged

Cat.No. : Car12-1958M
Product Overview : Purified recombinant protein of mouse full-length carbonic anhydrase 12 (Car12), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
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Description : This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 39.7 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Car12 carbonic anhydrase 12 [ Mus musculus (house mouse) ]
Official Symbol : Car12
Synonyms : CAR12; carbonic anyhydrase 12; carbonic anhydrase 12; CA XII; CA-XII; carbonic anhydrase XII; carbonate dehydratase XII; Ca12; AI314958; 2310047E01Rik
Gene ID : 76459
mRNA Refseq : NM_178396
Protein Refseq : NP_848483
UniProt ID : Q8CI85

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known inhibitors specific to CA12, and how do they impact its enzymatic function? 07/13/2021

Known inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.

In which specific cellular compartments is CA12 predominantly localized, and how does this localization influence its role? 06/12/2020

Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.

What role does CA12 play in tumorigenesis, especially in hypoxic tumor environments? 08/29/2019

CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.

What downstream signaling pathways are activated or inhibited by CA12? 07/28/2019

Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.

How does CA12 activity get modulated under varying pH conditions? 04/08/2018

The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.

How does CA12 expression vary in response to different cellular stressors or stimuli? 03/08/2018

Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.

How do genetic variants of the CA12 gene influence its protein structure and function? 10/18/2017

Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.

Customer Reviews (3)

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Reviews
12/20/2022

    Professional assistance, invaluable for complex protein experiments.

    05/09/2021

      Cost-effective quantitation, ideal for budget-conscious labs.

      11/21/2020

        Impressive expertise, accelerates our research progress.

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