Recombinant Mouse Car12 Protein, Myc/DDK-tagged

• Cat.No.: Car12-1958M
• Product Overview: Purified recombinant protein of mouse full-length carbonic anhydrase 12 (Car12), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 39.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Car12 carbonic anhydrase 12 [ Mus musculus (house mouse) ]
• Official Symbol: Car12
• Synonyms: CAR12; carbonic anyhydrase 12; carbonic anhydrase 12; CA XII; CA-XII; carbonic anhydrase XII; carbonate dehydratase XII; Ca12; AI314958; 2310047E01Rik
• Gene ID: 76459
• mRNA Refseq: NM_178396
• Protein Refseq: NP_848483
• UniProt ID: Q8CI85

Reviews

12/20/2022

Professional assistance, invaluable for complex protein experiments.

05/09/2021

Cost-effective quantitation, ideal for budget-conscious labs.

11/21/2020

Impressive expertise, accelerates our research progress.

Q&As

Are there any known inhibitors specific to CA12, and how do they impact its enzymatic function?

Known inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.

In which specific cellular compartments is CA12 predominantly localized, and how does this localization influence its role?

Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.

What role does CA12 play in tumorigenesis, especially in hypoxic tumor environments?

CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.

What downstream signaling pathways are activated or inhibited by CA12?

Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.

How does CA12 activity get modulated under varying pH conditions?

The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.

How does CA12 expression vary in response to different cellular stressors or stimuli?

Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.

How do genetic variants of the CA12 gene influence its protein structure and function?

Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.