"CA2" Related Products


Recombinant Mouse Car2 Protein, His-tagged

Cat.No.: Car2-886M
Product Overview: Recombinant mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.
Source: E. coli
Species: Mouse
Tag: His
Predicted N Terminal: Ser 2
Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
Molecular Mass: The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >96 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Car2 carbonic anhydrase 2 [ Mus musculus ]
Official Symbol: Car2
Synonyms: Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; CA II; CA-II; carbonate dehydratase II; carbonic anhydrase II
Gene ID: 12349
mRNA Refseq: NM_009801
Protein Refseq: NP_033931
UniProt ID: P00920
Chromosome Location: 3 A1; 3 3.23 cM
Pathway: Collecting duct acid secretion, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Pancreatic secretion, conserved biosystem
Function: arylesterase activity; carbonate dehydratase activity; metal ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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