Recombinant Mouse Car9 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Car9-914MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Car9 (NP_647466.2) extracellular domain (Met 1-Asp 390), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
• Protein length: 370
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Car9 carbonic anhydrase 9 [ Mus musculus ]
• Official Symbol: Car9
• Gene ID: 230099
• mRNA Refseq: NM_139305
• Protein Refseq: NP_647466

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.