Recombinant Mouse Ccl7 protein

Cat.No. : Ccl7-618M
Product Overview : Recombinant Mouse Ccl7 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic function for monocytes and eosinophils, but not for neutrophils.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 2× PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
Molecular Mass : Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
Protein length : 74
AA Sequence : QPDGPNASTCCYVKKQKIPKRNLKS YRRITSSRCPWEAVIFKTKKGMEVC AEAHQKWVEEAIAYLDMKTPTPKP
Endotoxin : Less than 1 EU/µg of rMuMCP-3/CCL7 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Ccl7
Official Symbol : Ccl7
Synonyms : CCL7; chemokine (C-C motif) ligand 7; C-C motif chemokine 7; RANTES/sis homolog; intercrine/chemokine MARC; small inducible cytokine A7; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3; fic; marc; mcp3; MCP-3; Scya7;
Gene ID : 20306
mRNA Refseq : NM_013654
Protein Refseq : NP_038682
UniProt ID : Q03366

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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