Recombinant Mouse CD28 Antigen, Fc-His

• Cat.No.: Cd28-1712M
• Product Overview: Recombinant Mouse CD28 (amino acids 1-149) was fused to the Fc region of human IgG1 (containing a 6x His-tag at the C-terminus) and expressed in NSO cells.

Specification

• Cat. No.: Cd28-1712M
• Description: CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-γ.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse CD28 is Mr 52 kDa. However, the actual molecular weight as observed by migration on SDS Page under reducing conditions is Mr 60 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Formulation: The protein was 0.2 μm filtered and lyophilized from PBS.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse CD28 was determined by its ability to inhibit IL2 secretion by stimulated Jurkat human leukemic T cells. The expected ED50 is 0.3-1.2 μg/ml when stimulated with 1 μg/ml rhB71/Fc Chimera in the presence of PHA.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Tag: His&Fc
• Protein length: 1-149

Gene Information

• Gene Name: Cd28 CD28 antigen [ Mus musculus ]
• Synonyms: Cd28; CD28 antigen; OTTMUSP00000002157; CH29-189K17.3
• Gene ID: 12487
• mRNA Refseq: NM_007642
• Protein Refseq: NP_031668
• UniProt ID: P31041
• Chromosome Location: 1 30.1 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Cell adhesion molecules (CAMs); Graft-versus-host disease; Systemic lupus erythematosus; T cell receptor signaling pathway; Type I diabetes mellitus

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.