Recombinant Mouse Cd55 Protein, His-tagged

• Cat.No.: Cd55-57M
• Product Overview: Recombinant mouse CD55, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: CD55, also known as Complement decay-accelerating factor, GPI-anchored, is a member of the RCA(regulators of complement activation) family characterized by four to 30 SCRs(short consensus repeats) in their plasma-exposed regions. It is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours.
• Source: Insect cells
• Species: Mouse
• Tag: His tag
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 36.8kDa (336aa)40-57kDa (SDS-PAGE under reducing conditions)
• AA Sequence: DCGPPPDIPN ARPILGRHSK FAEQSKVAYS CNNGFKQVPD KSNIVVCLEN GQWSSHETFC EKSCVAPERL SFASLKKEYL NMNFFPVGTIVEYECRPGFR KQPPLPGKAT CLEDLVWSPV AQFCKKKSCP NPKDLDNGHI NIPTGILFGS EINFSCNPGY RLVGVSSTFC SVTGNTVDWDDEFPVCTEIH CPEPPKINNG IMRGESDSYT YSQVVTYSCD KGFILVGNAS IYCTVSKSDV GQWSSPPPRC IEKSKVPTKK PTINVPSTGTPSTPQKPTTE SVPNPGDQPT PQKPSTVKVS ATQHVPVTKT TVRHPIRTST DKGEPNTGLE HHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Cd55 CD55 antigen [ Mus musculus ]
• Official Symbol: Cd55
• Synonyms: CD55; CD55 antigen; Daf; Daf1; Daf-GPI; GPI-DAF
• Gene ID: 13136
• mRNA Refseq: NM_010016
• Protein Refseq: NP_034146
• Pathway: Complement Activation, Classical Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Viral myocarditis, organism-specific biosystem
• Function: enzyme inhibitor activity

Reviews

02/23/2022

Efficient shipping and exceptional product quality. This company knows how to serve its customers.

09/20/2019

Consistency is key in research, and this product delivers that every time.

03/02/2018

We've tried various proteins, but this one stands out for its purity and performance.

Q&As

How do genetic variations in CD55 influence susceptibility to complement-mediated disorders, and what are the molecular mechanisms involved?

Genetic variations in CD55 may influence susceptibility to complement-mediated disorders by affecting the protein's ability to regulate complement activation. This can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH).

How does CD55 contribute to the modulation of immune responses beyond its role in complement regulation?

CD55 has immunomodulatory functions beyond complement regulation, influencing T cell responses and contributing to the maintenance of immune tolerance.

What role does CD55 play in protecting cells from complement-mediated lysis, and how does it contribute to immune evasion?

CD55 protects cells from complement-mediated lysis by preventing the formation of membrane attack complexes. This contributes to immune evasion by pathogens and self-cells alike.

Can you elaborate on the structural domains of CD55 and how they mediate its interactions with complement components?

CD55 consists of extracellular domains responsible for interactions with complement proteins, particularly C3b and C4b. These interactions prevent the formation of membrane attack complexes on host cells.

Can you discuss the involvement of CD55 in various diseases, including autoimmune disorders and infectious diseases?

CD55 dysregulation is associated with autoimmune disorders, where it may contribute to immune system overactivity. Additionally, some pathogens exploit CD55 to evade host immune responses.