Recombinant Mouse Cdk5rap3 Protein, Myc/DDK-tagged

• Cat.No.: Cdk5rap3-2090M
• Product Overview: Purified recombinant protein of mouse full-length CDK5 regulatory subunit associated protein 3 (Cdk5rap3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1. Required for liver development.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cdk5rap3 CDK5 regulatory subunit associated protein 3 [ Mus musculus (house mouse) ]
• Official Symbol: Cdk5rap3
• Synonyms: CDK5RAP3; CDK5 regulatory subunit associated protein 3; CDK5 regulatory subunit-associated protein 3; OK/SW-cl.114; C53; IC53; C81486; HSF-27; MST016; BC002318; MGC8303; 1810007E24Rik
• Gene ID: 80280
• mRNA Refseq: NM_030248
• Protein Refseq: NP_084524
• UniProt ID: Q99LM2