Recombinant Mouse Cnot7 Protein, Myc/DDK-tagged

• Cat.No.: Cnot7-2223M
• Product Overview: Purified recombinant protein of mouse full-length CCR4-NOT transcription complex, subunit 7 (Cnot7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
• Molecular Mass: 32.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cnot7 CCR4-NOT transcription complex, subunit 7 [ Mus musculus (house mouse) ]
• Official Symbol: Cnot7
• Synonyms: CNOT7; CCR4-NOT transcription complex, subunit 7; CCR4-NOT transcription complex subunit 7; CAF-1; POP2 homolog; CCR4-associated factor 1; catabolite repressor protein (CCR4)-associative factor 1; Caf1; Pop2; AU022737
• Gene ID: 18983
• mRNA Refseq: NM_011135
• Protein Refseq: NP_035265
• UniProt ID: Q60809