Recombinant Mouse CTCFL Protein (1-636 aa), His-SUMO-tagged

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Recombinant Mouse CTCFL Protein (1-636 aa), His-SUMO-tagged

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Cat.No. : CTCFL-434M
Product Overview : Recombinant Mouse CTCFL Protein (1-636 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.
Description : Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Ses to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
Source : E. coli
Species : Mouse
Tag : His-SUMO
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 89.1 kDa
Protein length : 1-636 aa
AA Sequence : MAAAEVPVPS GYFTQIKEQK LKPGDLEEEK EEDGVQRVEA QEGVVKEVEA ENSCLLLEAR APVESDRRIL TLQTVHLESQ DVHLQGLGWL SVPHSEELSG TVPEAEGILQ LPSVLWLDPE PQLSLQHCVT VSIPEELYPP EELQRIHFHL LRENVLMAEE NPELTPDLDE STALKKPEED EKDQLPPQGE TDKREERLLL LEMKPKEGKD DEIVLTISHL SLEEQQDPPA ANQTSVPGAK AAKPKRRRQT KGKPQSFQCD TCPFTSSKLS TFNRHIKIHS NERPHLCHLC LKAFRTVTLL RNHVNTHTGT RPHKCRDCDM AFVTSGELVR HRRYKHTYEK PFKCSLCKYA SVEASKMKRH IRSHTGERPF QCCQCAYASR DSYKLKRHMR THSGEKPYEC PTCHVRFTQS GTMKIHIAQK HGENVPKYEC PHCATIIARK SDLRVHLRNL HSQSPEEMKC RYCPAGFHER YALIQHQRTH KNEKKFKCKQ CDYACKQERC LKAHMRMHTG EKPFSCLACN KHFRQKQLLT VHLRKYHDPN FVPNLHLCLK CDKRFSRWSN LQRHRKKCDP EHETLAPNKD RRPVTRTQAS EGEAGHKEGE PQCPGEQALG HQGEAAGSQS PDHGLTCEII FNMMDK
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : Ctcfl CCCTC-binding factor (zinc finger protein)-like [ Mus musculus ]
Official Symbol : CTCFL
Synonyms : CTCFL; BORIS; CTCF paralog; Boris; RP23-44L6.1; OTTMUSG00000016680;
Gene ID : 664799
mRNA Refseq : NM_001081387
Protein Refseq : NP_001074856
UniProt ID : A2APF3

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