Recombinant Mouse CTCFL Protein (1-636 aa), His-tagged

• Cat.No.: CTCFL-1372M
• Product Overview: Recombinant Mouse CTCFL Protein (1-636 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
• Source: Yeast
• Species: Mouse
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 75.1 kDa
• Protein length: 1-636 aa
• AA Sequence: MAAAEVPVPSGYFTQIKEQKLKPGD LEEEKEEDGVQRVEAQEGVVKEVEA ENSCLLLEARAPVESDRRILTLQTV HLESQDVHLQGLGWLSVPHSEELSG TVPEAEGILQLPSVLWLDPEPQLSL QHCVTVSIPEELYPPEELQRIHFHL LRENVLMAEENPELTPDLDESTALK KPEEDEKDQLPPQGETDKREERLLL LEMKPKEGKDDEIVLTISHLSLEEQ QDPPAANQTSVPGAKAAKPKRRRQT KGKPQSFQCDTCPFTSSKLSTFNRH IKIHSNERPHLCHLCLKAFRTVTLL RNHVNTHTGTRPHKCRDCDMAFVTS GELVRHRRYKHTYEKPFKCSLCKYA SVEASKMKRHIRSHTGERPFQCCQC AYASRDSYKLKRHMRTHSGEKPYEC PTCHVRFTQSGTMKIHIAQKHGENV PKYECPHCATIIARKSDLRVHLRNL HSQSPEEMKCRYCPAGFHERYALIQ HQRTHKNEKKFKCKQCDYACKQERC LKAHMRMHTGEKPFSCLACNKHFRQ KQLLTVHLRKYHDPNFVPNLHLCLK CDKRFSRWSNLQRHRKKCDPEHETL APNKDRRPVTRTQASEGEAGHKEGE PQCPGEQALGHQGEAAGSQSPDHGL TCEIIFNMMDK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: Ctcfl CCCTC-binding factor (zinc finger protein)-like [ Mus musculus ]
• Official Symbol: CTCFL
• Synonyms: CTCFL; BORIS; CTCF paralog; Boris; RP23-44L6.1; OTTMUSG00000016680
• Gene ID: 664799
• mRNA Refseq: NM_001081387
• Protein Refseq: NP_001074856
• UniProt ID: A2APF3