Recombinant Mouse Cxcl16 protein

• Cat.No.: Cxcl16-85M
• Product Overview: Recombinant Mouse Cxcl16 protein was expressed in Escherichia coli.

Specification

• Description: CXCL16 is a member of the CXC chemokine family. Larger than other chemokines, it is one of the only two transmembrane chemokines in the family and the other is CX3CL1. Mouse CXCL16 has 246 a.a. and consists of a 26 a.a. residue putative signal peptide, an 88 a.a. residue chemokine domain, an 87 a.a. residue mucin-like spacer region, a 22 a.a. residue transmembrane domain, and a 23 a.a. residue cytoplasmic tail. Mouse CXCL16 shares 70 % sequence identity with human CXCL16 in chemokine domain. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. Functions of CXCL16 include inducing a strong chemotactic response and calcium mobilization. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.
• Source: E.coli
• Species: Mouse
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 20-1000 ng/ml.
• Molecular Mass: Approximately 9.9 kDa, a single non-glycosylated polypeptide chain containing 88 amino acids.
• Protein length: 88
• AA Sequence: NQGSVAGSCSCDRTISSGTQIPQGT LDHIRKYLKAFHRCPFFIRFQLQSK SVCGGSQDQWVRELVDCFERKECGT GHGKSFHHQKHLP
• Endotoxin: Less than 1 EU/μg of rMuCXCL16 as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: Cxcl16
• Official Symbol: Cxcl16
• Synonyms: CXCL16; chemokine (C-X-C motif) ligand 16; C-X-C motif chemokine 16; SR-PSOX/CXCL16; Cxc chemokine ligand 16; small-inducible cytokine B16; transmembrane chemokine CXCL16; zinc finger, MYND-type containing 15; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Zmynd15; AV290116; BB024863; CXCL16v1; CXCL16v2; 0910001K24Rik
• Gene ID: 66102
• mRNA Refseq: NM_023158
• Protein Refseq: NP_075647
• UniProt ID: Q8BSU2

Reviews

02/16/2022

Great protein crystallization services, pivotal for structural biology research.

05/09/2021

Swift protein sequencing, crucial for protein identification.

07/24/2018

Invaluable protein-protein interaction assays, integral to our research.

Q&As

How does CXCL16 function as both a chemokine and an adhesion molecule in immune cell recruitment and interaction?

CXCL16 acts as both a chemokine and adhesion molecule, guiding immune cell recruitment and facilitating cell-cell interactions.

How does CXCL16 influence the progression of inflammatory and autoimmune diseases?

CXCL16 influences inflammatory and autoimmune diseases, modulating immune cell recruitment and activation.

What is the impact of CXCL16 in tumor immunology, specifically in tumor infiltration by immune cells?

In tumor immunology, CXCL16 facilitates the infiltration of immune cells into tumors, affecting tumor immunity.

How do alterations in CXCL16 expression or signaling affect renal function and the progression of kidney diseases?

Changes in CXCL16 expression impact kidney function, potentially contributing to the development of renal diseases.

What role does CXCL16 play in the pathogenesis of atherosclerosis and cardiovascular diseases?

CXCL16 plays a role in atherosclerosis by recruiting immune cells to arterial walls, influencing plaque formation.

What are the mechanisms by which CXCL16 modulates the migration and activation of T cells and dendritic cells?

CXCL16 regulates T cell and dendritic cell migration and activation, playing a key role in immune responses.

How does CXCL16 contribute to the immune response against pathogens, particularly in viral infections?

CXCL16 boosts the immune response to pathogens, especially in viral infections, by attracting immune cells.