Recombinant Canine CXCL16 cell lysate

• Cat.No.: CXCL16-842CCL
• Product Overview: Canine CXCL16 / SRPSOX derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Canine
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CXCL16 chemokine (C-X-C motif) ligand 16 [ Canis lupus familiaris ]
• Official Symbol: CXCL16
• Synonyms: chemokine (C-X-C motif) ligand 16
• Gene ID: 607514
• mRNA Refseq: XM_844211
• Protein Refseq: XP_849304
• Chromosome Location: chromosome: 5
• Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem

Reviews

02/16/2022

Great protein crystallization services, pivotal for structural biology research.

05/09/2021

Swift protein sequencing, crucial for protein identification.

07/24/2018

Invaluable protein-protein interaction assays, integral to our research.

Q&As

How does CXCL16 function as both a chemokine and an adhesion molecule in immune cell recruitment and interaction?

CXCL16 acts as both a chemokine and adhesion molecule, guiding immune cell recruitment and facilitating cell-cell interactions.

How does CXCL16 influence the progression of inflammatory and autoimmune diseases?

CXCL16 influences inflammatory and autoimmune diseases, modulating immune cell recruitment and activation.

What is the impact of CXCL16 in tumor immunology, specifically in tumor infiltration by immune cells?

In tumor immunology, CXCL16 facilitates the infiltration of immune cells into tumors, affecting tumor immunity.

How do alterations in CXCL16 expression or signaling affect renal function and the progression of kidney diseases?

Changes in CXCL16 expression impact kidney function, potentially contributing to the development of renal diseases.

What role does CXCL16 play in the pathogenesis of atherosclerosis and cardiovascular diseases?

CXCL16 plays a role in atherosclerosis by recruiting immune cells to arterial walls, influencing plaque formation.

What are the mechanisms by which CXCL16 modulates the migration and activation of T cells and dendritic cells?

CXCL16 regulates T cell and dendritic cell migration and activation, playing a key role in immune responses.

How does CXCL16 contribute to the immune response against pathogens, particularly in viral infections?

CXCL16 boosts the immune response to pathogens, especially in viral infections, by attracting immune cells.