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Active Recombinant Mouse Cyp1b1 Protein

Cat.No. : Cyp1b1-123M
Product Overview : Recombinant mouse Cyp1b1 protein and CYP-reductase without tag were coexpressed in E. coli.
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Description : A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase)). Exhibits catalytic activity for the formation of hydroxyestrogens from 17beta-estradiol (E2), namely 2- and 4-hydroxy E2. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites. May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system. Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2. Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products. Plays an important role in retinal vascular development. Under ambient/hyperoxic O2 conditions, promotes angiogenesis and capillary morphogenesis of retinal endothelial cells and pericytes, likely by metabolizing the oxygenated products symptomatic of oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
Source : E. coli
Species : Mouse
Bio-activity : Cytochrome c Reductase Activity: 512 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: 7-ethoxyresorufin O-deethylase
Linear with time up to: 5 min(Determined at 0.1 μM substrate, using a P450 concentration of 1 pmol/ml)
Linear with P450 up to: 8 pmol/ml(Determined at 0.1 μM substrate, using an incubation time of 1 min)
Vmax: 17 pmol/min/pmol CYP
Km: 0.08 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 1.4 nmol/ml
Protein Concentration: 8.2 mg/ml
Specific P450 Content: 171 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 [ Mus musculus (house mouse) ]
Official Symbol : Cyp1b1
Synonyms : Cyp1b1; cytochrome P450, family 1, subfamily b, polypeptide 1; CP1B; CYPIB1; P4501b1; cytochrome P450 1B1; cytochrome P450, 1b1, benz[a]anthracene inducible; cytochrome P450CMEF; cytochrome P450EF; hydroperoxy icosatetraenoate dehydratase
Gene ID : 13078
mRNA Refseq : NM_009994
Protein Refseq : NP_034124
UniProt ID : Q64429

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CYP1B1 contribute to the metabolism of environmental toxins? 08/29/2022

It plays a key role in detoxifying environmental carcinogens and pollutants.

How does CYP1B1 interact with other cytochrome P450 enzymes? 07/24/2022

CYP1B1 works alongside other P450 enzymes, contributing to a broad spectrum of metabolic processes.

How do genetic variations in CYP1B1 affect its enzymatic function? 12/24/2021

Genetic mutations in CYP1B1 can lead to variations in metabolism, influencing drug response and toxicity.

What is the impact of altered CYP1B1 activity on cancer risk? 09/09/2021

Altered CYP1B1 activity is associated with an increased risk of certain cancers, due to its role in metabolizing carcinogens.

What potential therapeutic applications arise from targeting CYP1B1 in diseases? 03/22/2020

Targeting CYP1B1 offers potential for cancer prevention and treatment, particularly in managing hormone-related cancers.

What is the primary role of CYP1B1 in drug metabolism? 12/30/2017

CYP1B1, part of the cytochrome P450 family, is essential for metabolizing various drugs and chemicals.

What role does CYP1B1 play in steroid hormone synthesis? 07/07/2017

CYP1B1 is involved in the metabolism of steroid hormones, affecting their synthesis and breakdown.

Customer Reviews (3)

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03/15/2021

    Trustworthy data source. A critical research asset.

    02/15/2018

      Reliable partner in experiments. Enhances research quality.

      04/03/2017

        Dependable results. A research cornerstone.

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