Recombinant Mouse Dhdds Protein, Myc/DDK-tagged

• Cat.No.: Dhdds-2543M
• Product Overview: Purified recombinant protein of mouse full-length dehydrodolichyl diphosphate synthase (Dhdds), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: With NUS1, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length. Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 38.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dhdds dehydrodolichyl diphosphate synthase [ Mus musculus (house mouse) ]
• Official Symbol: Dhdds
• Synonyms: DHDDS; dehydrodolichyl diphosphate synthase; dedol-PP synthase; DS; HDS; W91638; 3222401G21Rik
• Gene ID: 67422
• mRNA Refseq: NM_026144
• Protein Refseq: NP_080420
• UniProt ID: Q99KU1