Recombinant Mouse Dihydrofolate Reductase, His-tagged

• Cat.No.: Dhfr-3422M
• Product Overview: Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 23.8 kDa (207aa) confirmed by MALDI-TOF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EF KYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KE PP RG AHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HL RL FV TRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Folate biosynthesis; One carbon pool by folate

Gene Information

• Gene Name: Dhfr dihydrofolate reductase [ Mus musculus ]
• Synonyms: dihydrofolate reductase; AA607882; AI662710; AW555094; 8430436I03Rik; Dhfr
• Gene ID: 13361
• mRNA Refseq: NM_010049
• Protein Refseq: NP_034179
• UniProt ID: P00375
• Chromosome Location: 13 C3; 13 43.0 cM
• Function: NADP or NADPH binding; dihydrofolate reductase activity; dihydrofolic acid binding; drug binding; oxidoreductase activity