Recombinant Mouse Dnmt1 Protein, Myc/DDK-tagged

• Cat.No.: Dnmt1-2620M
• Product Overview: Purified recombinant protein of mouse full-length DNA methyltransferase (cytosine-5) 1 (Dnmt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 183.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dnmt1 DNA methyltransferase (cytosine-5) 1 [ Mus musculus (house mouse) ]
• Official Symbol: Dnmt1
• Synonyms: DNMT1; DNA methyltransferase (cytosine-5) 1; DNA (cytosine-5)-methyltransferase 1; DNA MTase MmuI; DNA methyltransferase MmuI; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• Gene ID: 13433
• mRNA Refseq: NM_010066
• Protein Refseq: NP_034196
• UniProt ID: P13864