Active Recombinant Mouse FAM185A protein, His-tagged

• Cat.No.: FAM185A-5549M
• Product Overview: Recombinant Mouse FAM185A protein(Met1-Glu262), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-262 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured in a cell proliferation assay using mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng/mL, corresponding to a specific activity of 3.32×10^4~1.33×10^5 units/mg. 2. Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 8.31-33.24 ng/mL, corresponding to a specific activity of 3.01×10^4~1.20×10^5 units/mg.
• Molecular Mass: 30.19 kDa
• Endotoxin: < 0.01 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Fam185a family with sequence similarity 185, member A [ Mus musculus ]
• Official Symbol: FAM185A
• Synonyms: FAM185A; family with sequence similarity 185, member A; protein FAM185A; AI847670; 4631428I10
• Gene ID: 330050
• mRNA Refseq: NM_177869
• Protein Refseq: NP_808537