Active Recombinant Mouse Fgf2 protein(Ala11-Ser154), His-tagged

• Cat.No.: Fgf2-829M
• Product Overview: Recombinant Mouse Fgf2 (NP_032032.1) (Ala11-Ser154) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: N-His
• Protein length: Ala11-Ser154
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell prolifeRation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.
• Molecular Mass: The recombinant mouse Fgf2 consists 162 amino acids and predicts a molecular mass of 18.5 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgf2 fibroblast growth factor 2 [ Mus musculus ]
• Official Symbol: Fgf2
• Synonyms: FGF2; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2; Fgfb; bFGF; Fgf-2
• Gene ID: 14173
• mRNA Refseq: NM_008006
• Protein Refseq: NP_032032