| Species : |
Mouse |
| Source : |
CHO cells |
| Tag : |
Non |
| Protein Length : |
Ala253-Gly366 (mature protein, 114 aa) |
| Description : |
GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family. GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, white adipose tissue and the brain. The 366 amino acid mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein. Mature mouse GDF-3 shares 90% and 83% aa sequence identity with rat and human GDF-3, respectively. |
| Form : |
Lyophilized |
| Bio-activity : |
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. 50-100 ng/mL of Recombinant Mouse GDF-3 can effectively induce Smad2 phosphorylation. |
| Molecular Mass : |
13 kDa (predicted); 14-23 kDa, reducing conditions (SDS-PAGE); 16-18 kDa, non-reducing conditions |
| Endotoxin : |
<0.10 EU/ug (LAL method) |
| Purity : |
>90% by SDS-PAGE (Silver Staining and Coomassie Blue Staining) |
| Storage : |
-20 to -70 C as supplied; 2 to 8 C for 1 month after reconstitution; -20 to -70 C for 3 months after reconstitution |
| Reconstitution : |
Reconstitute at 100 ug/mL in 4 mM HCl |
| Reference : |
Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653 |
| Storage Buffer : |
Lyophilized from a 0.2 um filtered solution in HCl |
