Recombinant Mouse Gdf5 protein

• Cat.No.: Gdf5-651M
• Product Overview: Recombinant Mouse Gdf5 protein was expressed in Escherichia coli.

Specification

• Description: Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
• Source: E.coli
• Species: Mouse
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
• Molecular Mass: Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.
• Protein length: 120
• AA Sequence: APLANRQGKRPSKNLKARCSRKALH VNFKDMGWDDWIIAPLEYEAFHCEG LCEFPLRSHLEPTNHAVIQTLMNSM DPESTPPTCCVPTRLSPISILFIDS ANNVVYKQYEDMVVESCGCR
• Endotoxin: Less than 0.1 EU/µg of rMuGDF-5/BMP-14 as determined by LAL method.
• Purity: >96% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Gdf5
• Official Symbol: Gdf5
• Synonyms: GDF5; growth differentiation factor 5; growth/differentiation factor 5; GDF-5; brachypodism; cartilage-derived morphogenetic protein-1; bp; brp; Cdmp-1
• Gene ID: 14563
• mRNA Refseq: NM_008109
• Protein Refseq: NP_032135
• UniProt ID: P43027

Reviews

06/26/2022

Their dedicated team of experts is prompt, knowledgeable, and readily available to tackle any hurdles or concerns I may encounter.

03/20/2020

Used in protein electron microscopy structure analysis.Great.

08/31/2019

By harnessing the power of the GDF5 protein, I can harness its outstanding quality and tap into its potential to obtain dependable and meaningful findings.

Q&As

How does GDF5 contribute to cartilage repair?

GDF5 has been found to stimulate the proliferation and differentiation of chondrocytes, the cells responsible for cartilage formation and maintenance.

What preclinical evidence supports the use of GDF5 in orthopedics?

Preclinical studies have demonstrated GDF5's ability to promote bone and cartilage regeneration in animal models, providing a foundation for further clinical exploration.

How does GDF5 differ from other growth factors in terms of clinical applications?

GDF5's specificity for bone and cartilage development distinguishes it from other growth factors, making it a targeted option for orthopedic interventions.

Can GDF5 be used in combination with other therapies for enhanced effects?

Some studies suggest that combining GDF5 with other growth factors or therapeutic approaches may result in synergistic effects, improving overall outcomes.

Is GDF5 only relevant for bone and cartilage conditions, or are there other potential applications?

While its primary focus is on musculoskeletal conditions, GDF5's role in cellular differentiation opens avenues for exploring its applications in broader regenerative medicine contexts.