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Recombinant Mouse Gpc3 protein, Fc-tagged
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Cat.No. : | Gpc3-120M Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Mouse Gpc3 protein (Gln25-Met557), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293). |
Description : | Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. |
Source : | HEK293 |
Species : | Mouse |
Tag : | human IgG1 Fc |
Form : | Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
Molecular Mass : | This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 49.1 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | 533 |
Endotoxin : | Less than 1.0 EU per ug by the LAL method. |
Purity : | >90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
SDS-PAGE of Gpc3-120M : |
![]() Mouse Glypican 3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
Bioactivity-ELISA of Gpc3-120M : |
![]() Immobilized Human FGF basic, Tag Free at 2μg/mL (100 μL/well) can bind Mouse Glypican 3, Fc Tag with a linear range of 1.22-9.7 ng/mL (QC tested). |
Gene Name : | Gpc3 |
Official Symbol : | Gpc3 |
Synonyms : | GPC3; glypican 3; glypican-3; OCI-5 |
Gene ID : | 14734 |
mRNA Refseq : | NM_016697 |
Protein Refseq : | NP_057906 |
UniProt ID : | Q8CFZ4 |
◆ Recombinant Protein
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GPC3-5147H | Recombinant Human GPC3 Protein, GST-tagged | +Inquiry |
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Gpc3-272M | Recombinant Mouse Gpc3 Protein, His-tagged | +Inquiry |
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◆ Lysates
GPC3-2538HCL | Recombinant Human GPC3 cell lysate | +Inquiry |
GPC3-001MCL | Recombinant Mouse GPC3 cell lysate | +Inquiry |
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