Recombinant Mouse Gpc3 protein, Fc-tagged Gpc3-120M

Recombinant Mouse Gpc3 protein, Fc-tagged

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Recombinant Mouse Gpc3 protein, Fc-tagged

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Cat.No. : Gpc3-120M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Mouse Gpc3 protein (Gln25-Met557), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
Source : HEK293
Species : Mouse
Tag : human IgG1 Fc
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 49.1 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 533
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Gpc3-120M :

Mouse Glypican 3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of Gpc3-120M :

Immobilized Human FGF basic, Tag Free at 2μg/mL (100 μL/well) can bind Mouse Glypican 3, Fc Tag with a linear range of 1.22-9.7 ng/mL (QC tested).

Gene Name : Gpc3
Official Symbol : Gpc3
Synonyms : GPC3; glypican 3; glypican-3; OCI-5
Gene ID : 14734
mRNA Refseq : NM_016697
Protein Refseq : NP_057906
UniProt ID : Q8CFZ4
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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