| Description : |
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Source : |
Insect Cell |
| Species : |
Mouse |
| Bio-activity : |
Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml. |
| AA Sequence : |
Alpha chain: QKKRRNTLHEFKKSAKTTLTKEDPL LKIKTKKVNSADECANRCIRNRGFT FTCKAFVFDKSRKRCYWYPFNSMSS GVKKGFGHEFDLYENKDYIRNCIIG KGGSYKGTVSITKSGIKCQPWNSMI PHEHSFLPSSYRGKDLQENYCRNPR GEEGGPWCFTSNPEVRYEVCDIPQC SEVECMTCNGESYRGPMDHTESGKT CQRWDQQTPHRHKFLPERYPDKGFD DNYCRNPDGKPRPWCYTLDPDTPWE YCAIKTCAHSAVNETDVPMETTECI QGQGEGYRGTSNTIWNGIPCQRWDS QYPHKHDITPENFKCKDLRENYCRN PDGAESPWCFTTDPNIRVGYCSQIP KCDVSSGQDCYRGNGKNYMGNLSKT RSGLTCSMWDKNMEDLHRHIFWEPD ASKLNKNYCRNPDDDAHGPWCYTGN PLIPWDYCPISRCEGDTTPTIVNLD HPVISCAKTKQLR
Beta chain: VVNGIPTQTTVGWMVSLKYRNKHIC GGSLIKESWVLTARQCFPARNKDLK DYEAWLGIHDVHERGEEKRKQILNI SQLVYGPEGSDLVLLKLARPAILDN FVSTIDLPSYGCTIPEKTTCSIYGW GYTGLINADGLLRVAHLYIMGNEKC SQHHQGKVTLNESELCAGAEKIGSG PCEGDYGGPLICEQHKMRMVLGVIV PGRGCAIPNRPGIFVRVAYYAKWIH KVILTYKL |
| Endotoxin : |
Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg) |
| Purity : |
>95%, as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. |
| Storage Buffer : |
Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2 |
| Reconstitution : |
Resuspend the protein in the desired concentration in proper buffer. |
