| Description : |
This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| Source : |
E. coli |
| Species : |
Mouse |
| Bio-activity : |
The biological activity of murine IL-10 is measured by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells. The ED50 was found to be > 2 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg. |
| Molecular Mass : |
18.7 kDa |
| AA Sequence : |
MSRGQYSREDNNCTHFPVGQSHMLL ELRTAFSQVKTFFQTKDQLDNILLT DSLMQDFKGYLGCQALSEMIQFYLV EVMPQAEKHGPEIKEHLNSLGQKLK TLRMRLRRCHRFLPCENKSKAVEQV KSDFNKLEDQGVYKAMNEFDIFINC IEAYMMIKMKS |
| Endotoxin : |
Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg) |
| Purity : |
>95%, as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. |
| Storage Buffer : |
Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2 |
| Reconstitution : |
Resuspend the protein in the desired concentration in proper buffer. |
