Recombinant Mouse Il11 protein
|Product Overview :||Recombinant Mouse Il11 protein was expressed in Escherichia coli.|
- Gene Information
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|Description :||Interleukin-11 (IL-11) is encoded by the II11 gene. IL-11 is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. IL-11 is a member of the IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. IL-11 can directly stimulate the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induce megakaryocyte maturation resulting in increased platelet production. Mature murine IL-11 shares 88 % amino acid sequence identity with human IL-11.|
|Form :||Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.|
|Bio-activity :||Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine T11 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.|
|Molecular Mass :||Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.|
|Protein length :||179|
|AA Sequence :||MPGPPAGSPRVSSDPRADLDSAVLL TRSLLADTRQLAAQMRDKFPADGDH SLDSLPTLAMSAGTLGSLQLPGVLT RLRVDLMSYLRHVQWLRRAGGPSLK TLEPELGALQARLERLLRRLQLLMS RLALPQAAPDQPVIPLGPPASAWGS IRAAHAILGGLHLTLDWAVRGLLLL KTRL|
|Endotoxin :||Less than 1 EU/µg of rMuIL-11 as determined by LAL method.|
|Purity :||>97% by SDS-PAGE and HPLC analysis.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.|
|Reconstitution :||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.|
|Gene Name :||Il11|
|Official Symbol :||Il11|
|Synonyms :||IL11; interleukin 11; interleukin-11; IL-11;|
|Gene ID :||16156|
|mRNA Refseq :||NM_008350|
|Protein Refseq :||NP_032376|
|UniProt ID :||P47873|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.
Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.
Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.
Proteomics identifies IL11-interacting proteins and potential cross-talk partners.
Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.
RNA sequencing uncovers IL11-induced gene expression changes across different cell types.
X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.
Confocal and super-resolution microscopy visualize IL11 localization in tissues.
Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.
CRISPR screening unveils genes and pathways influenced by IL11 signaling.
Customer Reviews (3)Write a review
Potent in bone regeneration models. -
Suitable for cell culture. -
High yield in expression. -
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