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Recombinant Mouse Il12a protein, His & T7-tagged

Cat.No. : Il12a-851M
Product Overview : Recombinant Mouse Il12a aa. (Arg23~Ala215 (Accession # P43431)) fused with N-terminal His & T7 tag was produced in E. coli cells.
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  • Gene Information
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Source : E. coli
Species : Mouse
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 25.4kDa
Protein length : Arg23~Ala215 (Accession # P43431)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Characteristic : The isoelectric point is 8.2.
Applications : SDS-PAGE; WB; ELISA; IP
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4
Gene Name : Il12a interleukin 12a [ Mus musculus (house mouse) ]
Official Symbol : Il12a
Synonyms : Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; interleukin-12 subunit alpha; CLMF p35; IL-12 p35 subunit; IL-12 subunit p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12a p35 subunit; interleukin-12 p35 subunit
Gene ID : 16159
mRNA Refseq : NM_001159424.2
Protein Refseq : NP_001152896.1
UniProt ID : P43431

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do mass spectrometry-based techniques reveal post-translational modifications on IL12A? 08/18/2022

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models? 06/12/2022

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners? 06/26/2021

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture? 12/17/2020

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors? 10/25/2020

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells? 05/19/2019

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors? 06/05/2018

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function? 03/08/2018

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains? 11/23/2017

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms? 02/16/2017

Bioluminescence imaging tracks IL12A expression dynamics in vivo.

Customer Reviews (3)

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Reviews
01/14/2021

    Suitable for co-immunoprecipitation.

    12/21/2020

      Consistent in bioactivity.

      11/16/2020

        Good for receptor binding studies.

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