Recombinant Murine Interleukin 12a, P35-P40
Cat.No. : | Il12a-210M |
Product Overview : | Recombinant mouse IL-12 produced inSf9 insect cellsis a glycosylated disulfide-linked heterodimeric polypeptide chain of 506 amino acids having a molecular mass of 75 kDa consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits. It has been purified by using proprietary chromatographic techniques. It is expressed in Sf9 cells. MW=75kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Il12a-210M |
Description : | IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Recombinant mouse IL-12 produced in Sf9 insect cells is a glycosylated disulfide-linked heterodimeric polypeptide chain of 506 amino acids having a molecular mass of 75 kDa consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits. It has been purified by using proprietary chromatographic techniques. |
Source : | Sf9 cells. |
Purity : | Greater than 95% (determined by SDS-PAGE and HPLC analysis). |
Specific Activity : | NA. |
Endotoxin Level : | < 0.1 ng per µg of rMu IL-12-p40 (1EU/µg). |
Biological Activity : | The ED₅₀, as determined by the dose dependent secrtion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been co-stimulated with PMA, is 0.01- 0.05 ng/ml. |
Formulation : | Lyophilized from a sterile-filtered solution with no additives. |
Reconstitution : | Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity. |
Storage : | The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |
Gene Name : | Il12a interleukin 12a [ Mus musculus ] |
Synonyms : | Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232; interleukin 12a p35 subunit; interleukin 12a |
Gene ID : | 16159 |
mRNA Refseq : | NM_001159424 |
Protein Refseq : | NP_001152896 |
UniProt ID : | P43431 |
Chromosome Location : | 3 E1; 3 37.0 cM |
Pathway : | Allograft rejection; Cytokine-cytokine receptor interaction; RIG-I-like receptor signaling pathway; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus |
Function : | cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding |
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Il12a-47M | Active Recombinant Mouse Il12a Protein (Arg23-Ala215), C-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
IL12A-001CCL | Recombinant Cynomolgus IL12A cell lysate | +Inquiry |
IL12A-2435HCL | Recombinant Human IL12A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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