Recombinant Mouse Il17c Protein, Myc/DDK-tagged

• Cat.No.: Il17c-3500M
• Product Overview: Purified recombinant protein of mouse full-length interleukin 17C (Il17c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 22 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Il17c interleukin 17C [ Mus musculus (house mouse) ]
• Official Symbol: Il17c
• Synonyms: IL17C; interleukin 17C; IL-17C; MGC175740
• Gene ID: 234836
• mRNA Refseq: NM_145834
• Protein Refseq: NP_665833
• UniProt ID: Q8K4C5

Reviews

11/26/2021

High purity levels.

03/26/2021

Potent in anti-fungal assays.

06/07/2020

Suitable for receptor binding studies.

Q&As

How is the involvement of IL17C in autoimmune diseases elucidated using experimental models and patient samples?

Animal models, patient biopsies, and genetic association studies reveal IL17C's role in autoimmune diseases.

Can you explain the experimental designs used to study the crosstalk between IL17C and other cytokines in inflammatory responses?

Combination cytokine stimulation assays and signaling pathway inhibitors elucidate IL17C's crosstalk.

How is the function of IL17C in promoting tumor growth and progression investigated in cancer models?

Xenograft models and tumor growth measurements uncover IL17C's contribution to cancer progression.

How are the effects of IL17C modulation on specific immune cell populations characterized using flow cytometry and cell sorting?

Flow cytometry analysis with cell-specific markers characterizes IL17C's impact on immune cell subsets.

What molecular techniques are utilized to validate the therapeutic potential of targeting IL17C in inflammatory disorders?

Neutralizing antibodies and RNA interference validate IL17C's potential as a therapeutic target.

What approaches are taken to assess the role of IL17C in mucosal immunity and host defense against infections?

Infection models, bacterial challenges, and mucosal sampling assess IL17C's role in host defense.

Could you elaborate on the methods used to study IL17C's contribution to tissue-specific immune cell recruitment?

Chemotaxis assays and confocal microscopy study IL17C-mediated immune cell recruitment.

What strategies are used to explore the impact of IL17C on epithelial barrier integrity and inflammation?

Organoid culture systems and cytokine measurements explore IL17C's impact on epithelial barrier function.

What experimental techniques are employed to investigate the binding partners of IL17C and its downstream signaling pathways?

Co-immunoprecipitation and mass spectrometry identify IL17C interactors and signaling molecules.

How do researchers differentiate the functional roles of IL17C from other IL17 family members in immune responses?

Comparative functional assays and gene knockout models distinguish IL17C's unique roles.