Active Recombinant Mouse Il17f protein(Met1-Ala161), His-tagged

• Cat.No.: Il17f-964M
• Product Overview: Recombinant Mouse Il17f (NP_665855.2) (Met1-Ala161) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ala161
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20ng/mL TNFα. The ED50for this effect is 7-28ng/mL.
• Molecular Mass: The recombinant mouse Il17f consists 144 amino acids and predicts a molecular mass of 16.3 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il17f interleukin 17F [ Mus musculus ]
• Official Symbol: Il17f
• Synonyms: IL17F; interleukin 17F; interleukin-17F; C87042; IL-17F
• Gene ID: 257630
• mRNA Refseq: NM_145856
• Protein Refseq: NP_665855